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rice14 (r14) peptide  (GenScript corporation)

 
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    GenScript corporation rice14 (r14) peptide
    THP-1 macrophages were treated with <t>R14</t> peptide at concentrations ranged from 5–100 µM, or vehicle for 24 h (A), 48 h (B), and 72 h (C), and cell viability was determined by an MTT assay. Cells without the R14 peptide served as an untreated control. Values are expressed as mean ± SD of three independent experiments.
    Rice14 (R14) Peptide, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rice14 (r14) peptide/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    rice14 (r14) peptide - by Bioz Stars, 2026-06
    90/100 stars

    Images

    1) Product Images from "A wild rice-derived peptide R14 ameliorates monosodium urate crystals-induced IL-1β secretion through inhibition of NF-κB signaling and NLRP3 inflammasome activation"

    Article Title: A wild rice-derived peptide R14 ameliorates monosodium urate crystals-induced IL-1β secretion through inhibition of NF-κB signaling and NLRP3 inflammasome activation

    Journal: PeerJ

    doi: 10.7717/peerj.15295

    THP-1 macrophages were treated with R14 peptide at concentrations ranged from 5–100 µM, or vehicle for 24 h (A), 48 h (B), and 72 h (C), and cell viability was determined by an MTT assay. Cells without the R14 peptide served as an untreated control. Values are expressed as mean ± SD of three independent experiments.
    Figure Legend Snippet: THP-1 macrophages were treated with R14 peptide at concentrations ranged from 5–100 µM, or vehicle for 24 h (A), 48 h (B), and 72 h (C), and cell viability was determined by an MTT assay. Cells without the R14 peptide served as an untreated control. Values are expressed as mean ± SD of three independent experiments.

    Techniques Used: MTT Assay, Control

    Hemolytic activity of R14 peptide.
    Figure Legend Snippet: Hemolytic activity of R14 peptide.

    Techniques Used: Activity Assay, Control

    THP-1 macrophages were stimulated with MSU crystals (100 µg/mL) in the presence or absence of R14 peptide (5–100 µM) or colchicine (10 µM) for 6 h. The levels of IL-1β production were determined by sandwich ELISA. Values are expressed as mean ± SD of at least three independent experiments. ### p < 0.001 compared to the unstimulated THP-1 macrophages. * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with the MSU crystal-induced THP-1 macrophages.
    Figure Legend Snippet: THP-1 macrophages were stimulated with MSU crystals (100 µg/mL) in the presence or absence of R14 peptide (5–100 µM) or colchicine (10 µM) for 6 h. The levels of IL-1β production were determined by sandwich ELISA. Values are expressed as mean ± SD of at least three independent experiments. ### p < 0.001 compared to the unstimulated THP-1 macrophages. * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with the MSU crystal-induced THP-1 macrophages.

    Techniques Used: Sandwich ELISA

    THP-1 macrophages were stimulated with MSU crystals (100 µg/mL) in the presence or absence of R14 peptide (5–100 µM). After 6 h incubation, the protein expressions from supernatants and cell lysates were examined by Western blotting. The Western blot images of protein bands are the representative of three separate experiments (A). Bar diagrams showing densitometric analysis of the relative expression of pro-caspase-1/β-actin, intermediate caspase-1/β-actin, caspase-1/β-actin (B), pro-IL-1β/β-actin, IL-1β/β-actin (C) and NLRP3/β-actin (D), quantified using ImageJ software. Values are expressed as mean ± SD of three independent experiments. ## p < 0.01, ### p < 0.001 compared to the unstimulated THP-1 macrophages. * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with the MSU crystals-induced THP-1 macrophages.
    Figure Legend Snippet: THP-1 macrophages were stimulated with MSU crystals (100 µg/mL) in the presence or absence of R14 peptide (5–100 µM). After 6 h incubation, the protein expressions from supernatants and cell lysates were examined by Western blotting. The Western blot images of protein bands are the representative of three separate experiments (A). Bar diagrams showing densitometric analysis of the relative expression of pro-caspase-1/β-actin, intermediate caspase-1/β-actin, caspase-1/β-actin (B), pro-IL-1β/β-actin, IL-1β/β-actin (C) and NLRP3/β-actin (D), quantified using ImageJ software. Values are expressed as mean ± SD of three independent experiments. ## p < 0.01, ### p < 0.001 compared to the unstimulated THP-1 macrophages. * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with the MSU crystals-induced THP-1 macrophages.

    Techniques Used: Incubation, Western Blot, Expressing, Software

    THP-1 macrophages were stimulated with MSU crystals (100 µg/mL) in the presence or absence of R14 peptide (5–100 µM). After 30 min incubation, the expression of p-IκB-α and nuclear-NF-κB p65 protein were determined by Western blotting. The Western blot images of protein bands are the representative of three separate experiments. Bar diagrams showing densitometric analysis of the relative expression of p-IκB-α/β-actin (B) and nuclear NF-κB p65/β-actin (D), quantified using ImageJ software. Values are expressed as mean ± SD of three independent experiments. ## p < 0.01 compared to the unstimulated THP-1 macrophages. * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with the MSU crystals-induced THP-1 macrophages.
    Figure Legend Snippet: THP-1 macrophages were stimulated with MSU crystals (100 µg/mL) in the presence or absence of R14 peptide (5–100 µM). After 30 min incubation, the expression of p-IκB-α and nuclear-NF-κB p65 protein were determined by Western blotting. The Western blot images of protein bands are the representative of three separate experiments. Bar diagrams showing densitometric analysis of the relative expression of p-IκB-α/β-actin (B) and nuclear NF-κB p65/β-actin (D), quantified using ImageJ software. Values are expressed as mean ± SD of three independent experiments. ## p < 0.01 compared to the unstimulated THP-1 macrophages. * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with the MSU crystals-induced THP-1 macrophages.

    Techniques Used: Incubation, Expressing, Western Blot, Software

    THP-1 macrophages were stimulated with MSU crystals (100 µg/mL) in the presence or absence of R14 peptide (5–100 µM) for 6 h. The levels of ROS production were determined by measuring fluorescence at an excitation wavelength at 485 nm and an emission wavelength at 535 nm. Values are expressed as mean ± SD of three independent experiments. ### p < 0.001 compared to the unstimulated THP-1 macrophages. * p < 0.05 and ** p < 0.01 compared with the MSU crystal-induced THP-1 macrophages.
    Figure Legend Snippet: THP-1 macrophages were stimulated with MSU crystals (100 µg/mL) in the presence or absence of R14 peptide (5–100 µM) for 6 h. The levels of ROS production were determined by measuring fluorescence at an excitation wavelength at 485 nm and an emission wavelength at 535 nm. Values are expressed as mean ± SD of three independent experiments. ### p < 0.001 compared to the unstimulated THP-1 macrophages. * p < 0.05 and ** p < 0.01 compared with the MSU crystal-induced THP-1 macrophages.

    Techniques Used: Fluorescence



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    rice14 (r14) peptide  (GenScript corporation)
    90
    GenScript corporation rice14 (r14) peptide
    THP-1 macrophages were treated with <t>R14</t> peptide at concentrations ranged from 5–100 µM, or vehicle for 24 h (A), 48 h (B), and 72 h (C), and cell viability was determined by an MTT assay. Cells without the R14 peptide served as an untreated control. Values are expressed as mean ± SD of three independent experiments.
    Rice14 (R14) Peptide, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rice14 (r14) peptide/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    rice14 (r14) peptide - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    THP-1 macrophages were treated with R14 peptide at concentrations ranged from 5–100 µM, or vehicle for 24 h (A), 48 h (B), and 72 h (C), and cell viability was determined by an MTT assay. Cells without the R14 peptide served as an untreated control. Values are expressed as mean ± SD of three independent experiments.

    Journal: PeerJ

    Article Title: A wild rice-derived peptide R14 ameliorates monosodium urate crystals-induced IL-1β secretion through inhibition of NF-κB signaling and NLRP3 inflammasome activation

    doi: 10.7717/peerj.15295

    Figure Lengend Snippet: THP-1 macrophages were treated with R14 peptide at concentrations ranged from 5–100 µM, or vehicle for 24 h (A), 48 h (B), and 72 h (C), and cell viability was determined by an MTT assay. Cells without the R14 peptide served as an untreated control. Values are expressed as mean ± SD of three independent experiments.

    Article Snippet: Rice14 (R14) peptide at the purity of ≥ 95.0% (by HPLC) was synthesized by GenScript (Piscataway, NJ, USA).

    Techniques: MTT Assay, Control

    Hemolytic activity of R14 peptide.

    Journal: PeerJ

    Article Title: A wild rice-derived peptide R14 ameliorates monosodium urate crystals-induced IL-1β secretion through inhibition of NF-κB signaling and NLRP3 inflammasome activation

    doi: 10.7717/peerj.15295

    Figure Lengend Snippet: Hemolytic activity of R14 peptide.

    Article Snippet: Rice14 (R14) peptide at the purity of ≥ 95.0% (by HPLC) was synthesized by GenScript (Piscataway, NJ, USA).

    Techniques: Activity Assay, Control

    THP-1 macrophages were stimulated with MSU crystals (100 µg/mL) in the presence or absence of R14 peptide (5–100 µM) or colchicine (10 µM) for 6 h. The levels of IL-1β production were determined by sandwich ELISA. Values are expressed as mean ± SD of at least three independent experiments. ### p < 0.001 compared to the unstimulated THP-1 macrophages. * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with the MSU crystal-induced THP-1 macrophages.

    Journal: PeerJ

    Article Title: A wild rice-derived peptide R14 ameliorates monosodium urate crystals-induced IL-1β secretion through inhibition of NF-κB signaling and NLRP3 inflammasome activation

    doi: 10.7717/peerj.15295

    Figure Lengend Snippet: THP-1 macrophages were stimulated with MSU crystals (100 µg/mL) in the presence or absence of R14 peptide (5–100 µM) or colchicine (10 µM) for 6 h. The levels of IL-1β production were determined by sandwich ELISA. Values are expressed as mean ± SD of at least three independent experiments. ### p < 0.001 compared to the unstimulated THP-1 macrophages. * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with the MSU crystal-induced THP-1 macrophages.

    Article Snippet: Rice14 (R14) peptide at the purity of ≥ 95.0% (by HPLC) was synthesized by GenScript (Piscataway, NJ, USA).

    Techniques: Sandwich ELISA

    THP-1 macrophages were stimulated with MSU crystals (100 µg/mL) in the presence or absence of R14 peptide (5–100 µM). After 6 h incubation, the protein expressions from supernatants and cell lysates were examined by Western blotting. The Western blot images of protein bands are the representative of three separate experiments (A). Bar diagrams showing densitometric analysis of the relative expression of pro-caspase-1/β-actin, intermediate caspase-1/β-actin, caspase-1/β-actin (B), pro-IL-1β/β-actin, IL-1β/β-actin (C) and NLRP3/β-actin (D), quantified using ImageJ software. Values are expressed as mean ± SD of three independent experiments. ## p < 0.01, ### p < 0.001 compared to the unstimulated THP-1 macrophages. * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with the MSU crystals-induced THP-1 macrophages.

    Journal: PeerJ

    Article Title: A wild rice-derived peptide R14 ameliorates monosodium urate crystals-induced IL-1β secretion through inhibition of NF-κB signaling and NLRP3 inflammasome activation

    doi: 10.7717/peerj.15295

    Figure Lengend Snippet: THP-1 macrophages were stimulated with MSU crystals (100 µg/mL) in the presence or absence of R14 peptide (5–100 µM). After 6 h incubation, the protein expressions from supernatants and cell lysates were examined by Western blotting. The Western blot images of protein bands are the representative of three separate experiments (A). Bar diagrams showing densitometric analysis of the relative expression of pro-caspase-1/β-actin, intermediate caspase-1/β-actin, caspase-1/β-actin (B), pro-IL-1β/β-actin, IL-1β/β-actin (C) and NLRP3/β-actin (D), quantified using ImageJ software. Values are expressed as mean ± SD of three independent experiments. ## p < 0.01, ### p < 0.001 compared to the unstimulated THP-1 macrophages. * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with the MSU crystals-induced THP-1 macrophages.

    Article Snippet: Rice14 (R14) peptide at the purity of ≥ 95.0% (by HPLC) was synthesized by GenScript (Piscataway, NJ, USA).

    Techniques: Incubation, Western Blot, Expressing, Software

    THP-1 macrophages were stimulated with MSU crystals (100 µg/mL) in the presence or absence of R14 peptide (5–100 µM). After 30 min incubation, the expression of p-IκB-α and nuclear-NF-κB p65 protein were determined by Western blotting. The Western blot images of protein bands are the representative of three separate experiments. Bar diagrams showing densitometric analysis of the relative expression of p-IκB-α/β-actin (B) and nuclear NF-κB p65/β-actin (D), quantified using ImageJ software. Values are expressed as mean ± SD of three independent experiments. ## p < 0.01 compared to the unstimulated THP-1 macrophages. * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with the MSU crystals-induced THP-1 macrophages.

    Journal: PeerJ

    Article Title: A wild rice-derived peptide R14 ameliorates monosodium urate crystals-induced IL-1β secretion through inhibition of NF-κB signaling and NLRP3 inflammasome activation

    doi: 10.7717/peerj.15295

    Figure Lengend Snippet: THP-1 macrophages were stimulated with MSU crystals (100 µg/mL) in the presence or absence of R14 peptide (5–100 µM). After 30 min incubation, the expression of p-IκB-α and nuclear-NF-κB p65 protein were determined by Western blotting. The Western blot images of protein bands are the representative of three separate experiments. Bar diagrams showing densitometric analysis of the relative expression of p-IκB-α/β-actin (B) and nuclear NF-κB p65/β-actin (D), quantified using ImageJ software. Values are expressed as mean ± SD of three independent experiments. ## p < 0.01 compared to the unstimulated THP-1 macrophages. * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with the MSU crystals-induced THP-1 macrophages.

    Article Snippet: Rice14 (R14) peptide at the purity of ≥ 95.0% (by HPLC) was synthesized by GenScript (Piscataway, NJ, USA).

    Techniques: Incubation, Expressing, Western Blot, Software

    THP-1 macrophages were stimulated with MSU crystals (100 µg/mL) in the presence or absence of R14 peptide (5–100 µM) for 6 h. The levels of ROS production were determined by measuring fluorescence at an excitation wavelength at 485 nm and an emission wavelength at 535 nm. Values are expressed as mean ± SD of three independent experiments. ### p < 0.001 compared to the unstimulated THP-1 macrophages. * p < 0.05 and ** p < 0.01 compared with the MSU crystal-induced THP-1 macrophages.

    Journal: PeerJ

    Article Title: A wild rice-derived peptide R14 ameliorates monosodium urate crystals-induced IL-1β secretion through inhibition of NF-κB signaling and NLRP3 inflammasome activation

    doi: 10.7717/peerj.15295

    Figure Lengend Snippet: THP-1 macrophages were stimulated with MSU crystals (100 µg/mL) in the presence or absence of R14 peptide (5–100 µM) for 6 h. The levels of ROS production were determined by measuring fluorescence at an excitation wavelength at 485 nm and an emission wavelength at 535 nm. Values are expressed as mean ± SD of three independent experiments. ### p < 0.001 compared to the unstimulated THP-1 macrophages. * p < 0.05 and ** p < 0.01 compared with the MSU crystal-induced THP-1 macrophages.

    Article Snippet: Rice14 (R14) peptide at the purity of ≥ 95.0% (by HPLC) was synthesized by GenScript (Piscataway, NJ, USA).

    Techniques: Fluorescence